dako a0564 mouse anti sstr5 Search Results


sstr5  (Proteintech)
93
Proteintech sstr5
A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and <t>SSTR5</t> to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).
Sstr5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ad-cmv-marl13b-188  (VectorBuilder GmbH)
90
VectorBuilder GmbH ad-cmv-marl13b-188
A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and <t>SSTR5</t> to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).
Ad Cmv Marl13b 188, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5ht6 187  (Addgene inc)
93
Addgene inc 5ht6 187
A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and <t>SSTR5</t> to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).
5ht6 187, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dako+a0564+mouse+anti+sstr5/CMV-R-GECO1+(Plasmid+%2332444)/pm39939781-228-69-76
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rabbit anti-acetyl-α-tubulin (k40)  (BioNordika Oy)
90
BioNordika Oy rabbit anti-acetyl-α-tubulin (k40)
A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and <t>SSTR5</t> to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).
Rabbit Anti Acetyl α Tubulin (K40), supplied by BioNordika Oy, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ad cmv halo gli2 vectorbuilder vb240611 1532shj ad cmv marl13b 188 vectorbuilder custom  (Vector Biolabs)
95
Vector Biolabs ad cmv halo gli2 vectorbuilder vb240611 1532shj ad cmv marl13b 188 vectorbuilder custom
A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and <t>SSTR5</t> to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).
Ad Cmv Halo Gli2 Vectorbuilder Vb240611 1532shj Ad Cmv Marl13b 188 Vectorbuilder Custom, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dako+a0564+mouse+anti+sstr5/AAV-GFAP(0%2E7)-DIO-C1V1-TS-TdTomato/pm39939781-228-86-94
Average 95 stars, based on 1 article reviews
ad cmv halo gli2 vectorbuilder vb240611 1532shj ad cmv marl13b 188 vectorbuilder custom - by Bioz Stars, 2026-10
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Image Search Results


A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and SSTR5 to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).

Journal: bioRxiv

Article Title: Locally released somatostatin triggers cAMP and Ca 2+ signaling in primary cilia to modulate pancreatic β-cell function

doi: 10.1101/2024.06.05.597562

Figure Lengend Snippet: A. Confocal microscopy images showing ciliary localization of SSTR3 (green) in both insulin-positive and -negative cells in a human islet. B. Confocal microscopy images of a mouse islet showing the localization of SSTR3 and SSTR5 to primary cilia (visualized by anti-acetylated tubulin immunostaining and shown in magenta). C. Confocal microscopy images from human islets showing the localization of SSTR3 and SSTR5 to primary cilia. D. Confocal microscopy images of a MIN6 pseudo-islet showing the localization of SSTR3, but not SSTR5, to primary cilia. E. Quantifications of the effect of 18 h somatostatin treatment (100 nM) on cilia length in MIN6 pseudo-islets (means ± SEM; nctrl=111, nSST= 87 cilia from 3 different preparations, change in cilia length p=0.003 assessed by unpaired Kolmogorov-Smirnov test). F,G. Quantifications of the effect of 18h (G) and 15 min (H) somatostatin treatment on cilia SSTR3 immunoreactivity in MIN6 pseudo-islets (means ± SEM; for 18h SST treatments; n ctrl =111, n SST =87 cilia from 3 different preparations, change in SSTR3 not significant; for 15 min SST treatment n ctrl =340, n SST =295 cilia from 3 different preparations, P=0.0146. Significance is assessed by unpaired Kolmogorov-Smirnov test). STED microscopy images to the right show the distribution of SSTR3 (magenta) under resting condition and after 15 min SST stimulation. H. Quantification of fraction of SSTR3 positive cilia in pseudo-islets exposed to 100 nM somatostatin for 18h n ctrl =16 cilia from 3 islets, n SST =16 cilia from 4 islets. I,J,K. Quantifications of the effect of 18 h somatostatin treatment on cilia length (J), cilia SSTR3 immunoreactivity (K) and fraction of SSTR3-positive cilia (L) in mouse islet cells (means ± SEM; n ctrl =171, n SST =149 cilia from 2 different animals, P length =0.0055, P SSTR3 =0.0030, assessed by unpaired Kolmogorov-Smirnov test).

Article Snippet: The following primary antibodies were used in the study; acetylated tubulin (T745, host: mouse, 1:500; Sigma-Aldrich), Acetyl-α-Tubulin (K40) (5335, host: rabbit, 1:500; BioNordika), SSTR3 (E-AB-16077, host: rabbit, 1:200; Elabscience), Arl13b (ab136648, host: mouse, 1:300; Abcam), SST (A0566, host: rabbit, 1:500, Dako), Insulin (A0564, host: guinea pig, 1:500; Dako), SSTR5 (66772-1-Ig, host: mouse, 1:200, Proteintech), SSTR2 (ab134152, host: rabbit, 1:200, Abcam), Pericentrin (ab4448, Abcam, host: rabbit, 1:200), GABBR1 (AGB001AN102, Alomone labs, host: rabbit, 1:200), GLP-1R (Mab 7F38-s, Developmental Studies Hybridoma Bank, host: mouse, 1:10) and GFP (AB16901, Abcam, host: chicken, 1:500).

Techniques: Confocal Microscopy, Immunostaining, Microscopy

A. Confocal microscopy images of human islets from a non-diabetic and a type-2 diabetic (T2D) organ donor immunostained against insulin (cyan) and acetylated tubulin (magenta). B. Quantifications of cilia length in insulin positive and negative cells of non-diabetic and type-2 diabetic islets (ND: N=7 donors; 10-20 islets per donor; 946 cilia. T2D: N=5 donors; 10-20 islets per donor; 505 cilia; Student’t 2-tailed unpaired t-test). C. Number of cilia per area in islets from non-diabetic and type-2 diabetic islets (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). D. Length of cilia with a swollen tip morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=6 donors; T2D: n=5 donors; Mann-Whitney U-test). E. Length of cilia with rod-like morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). F. Fraction of cilia with a swollen tip morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). G. Correlation between cilium length and BMI in insulin positive (Ins+, top) and insulin negative (Ins-, bottom) cells in human islets from non-diabetic (black) and type-2 diabetic (red) donors (ND: n=6 donors; T2D: n=5 donors). H. Correlation between cilium length and donor age in insulin positive (Ins+, top) and insulin negative (Ins-, bottom) cells in human islets from non-diabetic (black) and type-2 diabetic (red) donors (ND: n=6 donors; T2D: n=5 donors). I. Confocal microscopy images of human islet from non-diabetic and type-2 diabetic donors immunostained against acetylated tubulin (green) and somatostatin (magenta). J. Number of δ-cells per islet area (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors; Student’s unpaired 2-tailed t-test). K. Shortest distance between primary cilia and δ-cells (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors; Student’s unpaired 2-tailed t-test). L. Correlation between number of δ-cells and shortest distance between δ-cells and primary cilia (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors). J. Confocal microscopy images of human islets from a non-diabetic and a type-2 diabetic donor immunostained against acetylated tubulin (magenta) and SSTR3 or SSTR5 (yellow).

Journal: bioRxiv

Article Title: Locally released somatostatin triggers cAMP and Ca 2+ signaling in primary cilia to modulate pancreatic β-cell function

doi: 10.1101/2024.06.05.597562

Figure Lengend Snippet: A. Confocal microscopy images of human islets from a non-diabetic and a type-2 diabetic (T2D) organ donor immunostained against insulin (cyan) and acetylated tubulin (magenta). B. Quantifications of cilia length in insulin positive and negative cells of non-diabetic and type-2 diabetic islets (ND: N=7 donors; 10-20 islets per donor; 946 cilia. T2D: N=5 donors; 10-20 islets per donor; 505 cilia; Student’t 2-tailed unpaired t-test). C. Number of cilia per area in islets from non-diabetic and type-2 diabetic islets (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). D. Length of cilia with a swollen tip morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=6 donors; T2D: n=5 donors; Mann-Whitney U-test). E. Length of cilia with rod-like morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). F. Fraction of cilia with a swollen tip morphology in human islets from non-diabetic and typ-2 diabetic donors (ND: n=7 donors; T2D: n=5 donors; Mann-Whitney U-test). G. Correlation between cilium length and BMI in insulin positive (Ins+, top) and insulin negative (Ins-, bottom) cells in human islets from non-diabetic (black) and type-2 diabetic (red) donors (ND: n=6 donors; T2D: n=5 donors). H. Correlation between cilium length and donor age in insulin positive (Ins+, top) and insulin negative (Ins-, bottom) cells in human islets from non-diabetic (black) and type-2 diabetic (red) donors (ND: n=6 donors; T2D: n=5 donors). I. Confocal microscopy images of human islet from non-diabetic and type-2 diabetic donors immunostained against acetylated tubulin (green) and somatostatin (magenta). J. Number of δ-cells per islet area (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors; Student’s unpaired 2-tailed t-test). K. Shortest distance between primary cilia and δ-cells (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors; Student’s unpaired 2-tailed t-test). L. Correlation between number of δ-cells and shortest distance between δ-cells and primary cilia (ND: 31 islets, 3 donors; T2D: 32 islets, 2 donors). J. Confocal microscopy images of human islets from a non-diabetic and a type-2 diabetic donor immunostained against acetylated tubulin (magenta) and SSTR3 or SSTR5 (yellow).

Article Snippet: The following primary antibodies were used in the study; acetylated tubulin (T745, host: mouse, 1:500; Sigma-Aldrich), Acetyl-α-Tubulin (K40) (5335, host: rabbit, 1:500; BioNordika), SSTR3 (E-AB-16077, host: rabbit, 1:200; Elabscience), Arl13b (ab136648, host: mouse, 1:300; Abcam), SST (A0566, host: rabbit, 1:500, Dako), Insulin (A0564, host: guinea pig, 1:500; Dako), SSTR5 (66772-1-Ig, host: mouse, 1:200, Proteintech), SSTR2 (ab134152, host: rabbit, 1:200, Abcam), Pericentrin (ab4448, Abcam, host: rabbit, 1:200), GABBR1 (AGB001AN102, Alomone labs, host: rabbit, 1:200), GLP-1R (Mab 7F38-s, Developmental Studies Hybridoma Bank, host: mouse, 1:10) and GFP (AB16901, Abcam, host: chicken, 1:500).

Techniques: Confocal Microscopy, MANN-WHITNEY